三維分析缺血性卒中大鼠內(nèi)皮卒中后血管生成
染色方法：
熒光染料標記
標記方法：
lectin-FITC Lectin-DyLight 594
包埋方法：
樹脂包埋
成像平臺：
BioMapping 5000
Figure 1. Inhibiting Sema3E/PlexinD1 signal- ing improves vascular network recovery after tMCAO. A) Schematic illustration of treatment with oriented injection of LV-mediated Plex- inD1-specific shRNA (sh) or control shRNA into ischemic cortex. B) The representative reconstructed images of cerebral vascular network by MOST at d 14 after tMCAO. Original magnification value, 35. C) Blood vessel density of in perilesion area of recon- structed images was quantified. Data are presented as means 6 SEM ; n >= 3. *P , 0.05, ***P , 0.001 vs. control or indicated group.
Figure 1. Inhibiting Sema3E/PlexinD1 signal- ing improves vascular network recovery after tMCAO. A) Schematic illustration of treatment with oriented injection of LV-mediated Plex- inD1-specific shRNA (sh) or control shRNA into ischemic cortex. B) The representative reconstructed images of cerebral vascular network by MOST at d 14 after tMCAO. Original magnification value, 35. C) Blood vessel density of in perilesion area of recon- structed images was quantified. Data are presented as means 6 SEM ; n >= 3. *P , 0.05, ***P , 0.001 vs. control or indicated group.
2019年4月3日，華中科技大學同濟醫(yī)學院附屬協(xié)和醫(yī)院神經(jīng)內(nèi)科胡波教授課題組，使用MOST技術(shù)來充分展示包括毛細血管在內(nèi)的血管模式的細節(jié)，揭示了在短暫的大腦中動脈閉塞后，抑制 PlexinD1信號導致缺血區(qū)血管網(wǎng)有組織地恢復的可能機制。文章發(fā)表在《FASEB》雜志。
參考文獻
參考文獻[1]:Zhou YF et al., Sema3E/PlexinD1 signaling inhibits postischemic angiogenesis by regulating endothelial DLL4 and filopodia formation in a rat model of ischemic stroke. FASEB 2019 Jan 17
武漢東湖新技術(shù)開發(fā)區(qū)豹澥街道高科園三路9號武漢光谷精準醫(yī)療產(chǎn)業(yè)基地2.1期13棟12層